We did compare the migration patterns of SMOBIOs Protein Markers with other brands, and we concluded that it was difficult to define precision due to the reasons mentioned above. For example, a protein which is highly hydrophilic might show a particular higher position in the SDS-PAGE analysis when compared to a hydrophobic one. It is known that the analysis of protein size by an SDS-PAGE is only for estimation because of the intrinsic variation of amino acid composition in all proteins including stained and non-stained ones. ![]() Usually, pre-stained marker is written on estimated molecular weight for caution. No need to mark the bands with a pencil, no need for any additional treatment before the secondary antibody! Do You Have Data Comparison For Protein Molecular Weights Precision With Other Protein Markers All markers are bound by the secondary antibody and the bands are clearly visible. The AcuteBand Pre-stained Protein Ladder is also ideal for Western Blotting by the LICOR System.
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